Aiming at the cross-layer interference between the macrocell user layer and the femtocell user layer and the same layer interference between femtocells in the macrocell-femtocell dual-layer network model, a resource allocation algorithm based on femtocell base stations was proposed. The algorithm consists of two parts:the one is that, the Macrocell base station firstly used the improved difference method, set the virtual Macro User Equipment (MUE), and turned it into a balanced assignment problem and allocated the channel to the macrocell user, and then used water-filling algorithm for power allocation to ensure the transmission of macrocell users. The other part is that, on the basis of guaranteeing the service quality of the macrocell users, an Enhanced Ant Colony Optimization (EACO) algorithm was adopted to group the femtocells after setting the pheromone concentration range, which avoided the possibility that the original ant colony algorithm may fall into a local optimum. Then, a heuristic algorithm and a distributed power allocation algorithm were used to allocate the channel and power to the Femto User Equipment (FUE) respectively. The spectral efficiency was maximized under the data rate requirement of the femtocell users. The simulation results show that EACO effectively suppresses cross-layer interference and same-level interference, which can guarantee the data rate requirement of users and improve the efficiency of network spectrum effectively.